![]() ![]() Changes in spreading and cytoskeleton organization of glaucoma LC cells were significantly more pronounced than those in normal cells. These changes were positively associated with elevated cell stiffness measured by AFM. Significantly greater cell spread area along with increased actin filament development, and vinculin-focal adhesion formation (number and size) were found in both normal and glaucoma LC cells cultured on stiff substrates. The elastic modulus of cells was measured using atomic force microscopy (AFM). Cell morphology, spread area, actin stress fibers, vinculin-focal adhesion formation, and α-smooth muscle actin (α-SMA) signal were examined using immunofluorescence staining. Primary human LC cells from normal and age-matched POAG glaucoma donors were cultured on substrates with defined mechanical properties of 5 and 100 kPa to replicate the range of mechanical microenvironments that cells may experience in vivo. Here using primary human LC cells, we investigate the effect of substrate stiffness on profibrotic changes, which might be a key molecular mechanism driving ECM remodeling of the LC in primary open-angle glaucoma (POAG) glaucoma. Increased ECM tissue stiffness drives myofibroblast activation leading to tissue fibrosis throughout the body. So if you’re looking to engage in rewarding research that’s having a positive global impact – apply for a PhD with Monash IT today.Alteration in the extracellular matrix (ECM) of the optic nerve head (ONH) causes lamina cribrosa (LC) fibrosis and affects the mechanical integrity of the ONH. Pioneers in AI, data science, cybersecurity, digital health, software systems and human-centred computing.Our students also enjoy access to cutting-edge facilities, such as our studios in the Woodside Building for Technology and Design, as well as a worldwide network of international and academic partners. Drive eye-opening projects with leading experts A destination of choice for graduate research, all our exceptional PhD candidates undertake innovative social good projects while collaborating with world-class specialists. The funds can be used for travel, fieldwork, data collection, specialised training and equipment. Our faculty also covers overseas health cover and allocates living stipends to PhD students studying in Australia. Every research initiative is eligible – and we automatically put you forward for potential scholarships. We also have dedicated options for women in IT, low socio-economic status and other underrepresented groups, as well as funding for Indigenous Australian students. Graduate research – with less financial stress We offer many generous scholarships to domestic and international candidates, and are open to self-funded ones. ![]() Out of the prestigious Group of Eight, Monash is the only university to have a dedicated IT faculty.We’re also rated ‘Above world standard’ across all categories in Information and Computing Sciences, ranked five out of five for AI and Image Processing** and we’re home to the largest group of data scientists of any research institution in the southern hemisphere. Study at Australia’s most innovative university* – and gain deep, unrivalled expertise in a discipline within IT and Computer Science. ![]()
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